i have done some research on this topic. Not sure if my opinion is correct. Pls give comment.
I've looked into the journals about the storage of the cord blood cells and found at present, no consenses has been reached how to store best.
Most of the research concentrated on how to extract the stem cells and what kind of additives they added to the stem cells determine the viability of the cells when they thraw (defrost). Most of them said the cells are likely to survive if the cells are gradually frozened by the temperature of 1-5C/min rather than to quickly frozened to the target temperature. Centrifucation (as in Cordlife, not sure in the other 2 companies) is the worst method to separate the stem cells from the rest of the cord blood.
The additive of the storage was very important as it helps to avoid the sudden shrinkage and swelling of the cells and thus help them to survive during the freezing and defrose procedures.
The problem of cryolife of using the "tubes" in storing the cells is not what we worry that the bacterial may get inside. 1 study did a test to check what would happen if environmental bacteria contaminated the cell. The results showed although the bacteria still present after the defrose procedures, the amount of it is not large enough to make the cell not usable.
The problem of the tube was that 1 study had compared different containers: polyethene bag, another material maded bag and tubes and found the no. of stem cells remained alive is much less then the first 2 material.
I still need some more time to research in this aspect.
As regard to the sudden change of temperature, if the appropriate additive is added there is not much difference between the change in a bit in temperature, as -196 is just a convenient temp the researcher got as it is the temperature when the liquid nitrogen became gas. So i think all 3 companies actually doesn't make much difference in this aspect.
So, if any mother have chance to go to their company for a visit, please ask these questions and i am longing for the answers as well.
I have gone though all of you's response. I find that it's hard for me to choose a company based on your opinion since most of the comments offered seem biased or coming from the sales of the company concerned.:lol: :lol:
Be a smart mother, I think I have to spend sometime to visit the lab. of the company by myself before going to a decision.
原文章由 DaisyDaisy 於 07-8-27 17:53 發表 I have gone though all of you's response. I find that it's hard for me to choose a company based on your opinion since most of the comments offered seem biased or coming from the sales of the com ...
I have joined Cyrolife. I have contacted both the sales guy of Cyrolife, Cordlife and Healthbaby, but seems Cyrolife is more professional and easy going although it is most expensive.
Cell Therapy Technologies Centre Ltd. (it is Cryolife's lab name in HK and the location is also in HK)
But for the other 2 companies, only there mother companies have the AABB certified.
The sales said if in case there is any problem they cannot save the cord blood, all paid money will be refund. Or after several year we have used or donated the blood, that means no need to store it, the balance payment will be refund also.
The main reason I joined Cyrolife is because it is recommended by my Doctor.
There is a referral discount. If needed, you can PM me and I provide you my ID.