Elenalan 發表於 22-9-7 11:39
我一向有睇學術期刊，上年11月睇到呢個早期關於新冠滅活疫苗研究 (同濟大學) - full version可網上下載。
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Abstract
Large-scale COVID-19 vaccinations are currently underway in many countries in response to the COVID-19 pandemic.
Here, we report, besides generation of neutralizing antibodies, consistent alterations in hemoglobin A1c, serum
sodium and potassium levels, coagulation profiles, and renal functions in healthy volunteers after vaccination with an
inactivated SARS-CoV-2 vaccine. Similar changes had also been reported in COVID-19 patients, suggesting that
vaccination mimicked an infection. Single-cell mRNA sequencing (scRNA-seq) of peripheral blood mononuclear cells
(PBMCs) before and 28 days after the first inoculation also revealed consistent alterations in gene expression of many
different immune cell types. Reduction of CD8+ T cells and increase in classic monocyte contents were exemplary.
Moreover, scRNA-seq revealed increased NF-κB signaling and reduced type I interferon responses, which were
confirmed by biological assays and also had been reported to occur after SARS-CoV-2 infection with aggravating
symptoms. Altogether, our study recommends additional caution when vaccinating people with pre-existing clinical
conditions, including diabetes, electrolyte imbalances, renal dysfunction, and coagulation disorders.
Elenalan 發表於 22-9-7 11:39
我一向有睇學術期刊，上年11月睇到呢個早期關於新冠滅活疫苗研究 (同濟大學) - full version可網上下載。
...
Discussion
This is a comprehensive investigation of the patho-
physiological changes, including detailed immunological
alterations in people after COVID-19 vaccination. Results
indicated that vaccination, in addition to stimulating the
generation of neutralizing antibodies, also influenced
various health indicators including those related to dia-
betes, renal dysfunction, cholesterol metabolism, coagu-
lation problems, electrolyte imbalance, in a way as if the
volunteers experienced an infection. scRNA-seq of
PBMCs from volunteers before and after vaccination
revealed dramatic changes in immune cell gene expres-
sion, not only echoing some of the clinical laboratory
measures but also suggestive of increased NF-κB-related
inflammatory responses, which turned out to be mainly
taking place in classical monocytes. Vaccination also
increased classical monocyte contents. Moreover, the
gene set positively contributing to MVS scores, also
known to be associated with severe symptom develop-
ment, was highly expressed in monocytes. Type I inter-
feron (IFN-α/β) responses, supposedly beneficial against
COVID-19, were downregulated after vaccination. In
addition, the negative MVS genes were highly expressed
in lymphocytes (T, B, and NK cells), yet showed reduced
expression after vaccination. Together, these data sug-
gested that after vaccination, at least by day 28, other than generation of neutralizing antibodies, people’s immune
systems, including those of lymphocytes and monocytes,
were perhaps in a more vulnerable state.
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